The temperature-insensitive extracted from a bacterium found in hot springs can withstand the high temperatures needed to the double-stranded dna and can continue uninterrupted.

The correct answer will be:

1. Taq polymerase enzyme

2. separate

3. replication

Explanation:

Taq polymerase is an enzyme used in the Polymerase chain reaction (PCR) technique to make new strands form the existing strands.

This enzyme belongs to "type I" family of DNA polymerase enzyme which can withstand the high temperature which is required to separate the double-stranded DNA. This enzyme was extracted from a heat-tolerant bacterium called Thermus aquaticus in 1976.  

Thus, these options are the correct answer.