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Business, 23.08.2021 20:20 kaysayfam747

In this experiment you want to determine the nature of the membrane protein. You compare untreated control cells (top
lane) to cells treated with trypsin (bottom).
After trypsin treatment you run the proteins out on an
SDS-PAGE (the top (+) of the gel is to the left) and transfer
them to a membrane.
You are interested in 5 different proteins for which you
have specific antibodies. You do westerns to identify the 5
proteins.
What type of membrane protein is protein #2?
(look at image lec 4-6)
A. A TM protein with a large domain on the outside
B. A TM protein with large domain inside the cell
C. A TM protein with domains both inside and outside the cell
D. A peripheral cytoplasmic protein
E. A peripheral extracellular protein

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