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Chemistry, 01.07.2019 21:20 hardwick744

3. solving the sequence of an oligopeptide from sequence analysis data amino acid analysis of a decapeptide revealed the presence of the following products: nh4 1 asp glu tyr arg met pro lys ser phe the following facts were observed: a. neither carboxypeptidase a nor b treatment of the decapeptide had any effect. b. trypsin treatment yielded two tetrapeptides and free lys. c. clostripain treatment yielded a tetrapeptide and a hexapeptide. d. cyanogen bromide treatment yielded an octapeptide and a dipeptide of sequence np (using the one-letter codes). e. chymotrypsin treatment yielded two tripeptides and a tetrapeptide. the n-terminal chymotryptic peptide had a net charge of 21 at neutral ph and a net charge of 23 at ph 12. f. one cycle of edman degradation gave the pth derivative

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