If you were setting up an experiment to disprove spontaneous generation in a liquid medium, which of the following would be essential to the experiment?
a) supplying the liquid with nutrients
b) starting with a liquid that contains microorganisms
c) adding antibiotics to the liquid
d) using a sterile liquid and eliminating exposure to microorganisms
e) adding carbon dioxide to the liquid

D.- Using a sterile liquid and eliminating exposure to microorganisms.

Explanation:

Remember that Redi´s experiment is going to be with different jars cover the top. In this case, if the liquid is sterile that means any living thing is present there. so if by passing the time, there are no microorganisms, this is going to be the results that the spontaneous generation does not exist. From a non living liquid there can not produce a living thing.

a electronegativity decreases beacuse the size of nucleus is bigger.