Peptides can be separated using an ion‑exchange column based on their isoelectric (pi) values. at which ph values would two different peptides, one with a pi of 5.5 and the other with a pi of 9.1 , bind to a cation‑ and anion‑exchange column? each peptide may be capable of binding to each column at more than one ph value.

The independent variable is the type of music.the dependent variable is the pulse rate.the intervening variable is the age of the subject.if the mother does not like rock, her pulse is likely to not increase.the same might be true for the sister.the friend may like rap and gets excited.to establish cause/effect relationship, all of the subjects must be consistent.hope i .

The breaking apart of glucose, which involves spontaneous reactions