Using the hh eq. calculate the amounts (masses) of sodium phosphate monobasic monohydrate (nah2po4 h2o) and sodium phosphate dibasic anhydrous (na2hpo4) required to prepare 200 ml of a 100 mm phosphate buffer at ph 6.24. assume a pk, of 7.2 for the monobasic phosphate. this buffer will be used in the activity assay of our purified lysozyme in a few short weeks. again scan/photo a copy of your work, or use equation editor/word to show calculations.

7. using your knowledge of colligative properties explain whether sodium chloride or calcium chloride would be a more effective substance to melt the ice on a slick sidewalk. use 3 – 4 sentences in your explanation. 8. when a 2.5 mol of sugar (c12h22o11) are added to a certain amount of water the boiling point is raised by 1 celsius degree. if 2.5 mol of aluminum nitrate is added to the same amount of water, by how much will the boiling point be changed? show all calculations leading to your answer or use 3 – 4 sentences to explain your answer. 9. if 5.40 kcal of heat is added to 1.00 kg of water at 100⁰c, how much steam at 100⁰c is produced? show all calculations leading to an answer. 10. the freezing of water at 0⁰c can be represented as follows: h2o (l) ↔ h2o(s) the density of liquid water is 1.00 g/cm3. the density of ice is 0.92 g/cm3. in 3 – 4 sentences explain why applying pressure causes ice to melt.