Add 100 ml of 0.1 m solution of amino acid a to a 250-ml beaker. obtain a magnetic stir-bar from your instructor and place it in the beaker. 3. add 1.0m naoh to a clean 50-ml buret. fill up the buret and drain some of the solution out so that the tip of the buret is filled (make sure there is no air bubbles in the tip) 4. place the beaker with the amino acid solution in it directly in the center of the stir-plate. turn on the stirrer and get the stir-bar stirring at a comfortable rate, where no solution is splashing out of the beaker 5. carefully adjust the position of the magnetic stir-bar to prevent hitting the electrode of the ph meter. the electrode will remain in the solution throughout the experiment 6. record the ph of the amino acid solution before adding any 1.0m naoh 7. start the titration by adding 0.5ml 1.0m naoh, with stirring, and record the ph when the meter is stable. repeat the addition of 0.5ml 1.0m naoh and record the resulting ph until the ph reaches 12 if you accidentally add extra 1.0m naoh record the exact amount of naoh added. 8. repeat the step 2 - 7 for the second unknown. calculate the millequivalents (meq) of base necessary for half neutralization of the ammonium and carboxyl groups. meq. of a base is the equivalent amount of oh-that react with the amino acid during titration. the given concentration of both amino acid solutions is 0.1 m. if 0.5 ml of 1.0 m naoh is added to titrate the amino acid, the meq. of oh will be 0.05 because only 0.05 mmol of naoh reacts with the amino acid.