Chymotrypsin is a serine protease enzyme that we talked about in class. in order for chymotrypsin to function, his-57 in the active site must be deprotonated. at ph = 7, approximately what percentage of chymotrypsin proteins are active? use the henderson-hasselbalch equation provided and assume the pka of his is 6.

Percentage of chymotrypsin proteins that are active is 90,91%

Explanation:

The active site, His-57, is in equilibrium, thus:

His-57H⁺ ⇄ His-57 + H⁺

Where His-57H⁺ is the active site in its protonated form.

For this equilibrium, Henderson-Hasselbalch formula is:

pH = pka + log₁₀ (His-57/His-57H⁺)

If pH is 7 and pka is 6:

7 = 6 + log₁₀ (His-57/His-57H⁺)

1 = log₁₀ (His-57/His-57H⁺)

10 = His-57/His-57H⁺

10 (His-57H⁺) = His-57 (1)

If total active sites are 100%

100 = His-57H⁺ + His-57 (2)

Replacing (2) in (1)

His-57H⁺ = 9,09%

His-57 = 90,91%

Thus, percentage of chymotrypsin proteins that are active is 90,91%

I hope it helps!

explanation:

two years ago, when picking flowers in the mountains back of yosemite valley, i found a book. it was blotted and storm-beaten; all of its outer pages were mealy and crumbly. the paper seemed to dissolve like the snow beneath which it had been buried, however; many of the inner pages were well preserved. and although all were more or less stained and torn, whole chapters were easily readable. this is the condition of the great open book of yosemite glaciers today; its granite pages have been torn and blurred by the same storms that wasted the castaway book.

two years ago, when picking flowers in the mountains back of yosemite valley; i found a book. it was blotted and storm-beaten, all of its outer pages were mealy and crumbly. the paper seemed to dissolve like the snow beneath which it had been buried, however, many of the inner pages were well preserved. and although all were more or less stained and torn; whole chapters were easily readable. this is the condition of the great open book of yosemite glaciers today, its granite pages have been torn and blurred by the same storms that wasted the castaway book.