Ipropose to design a new drug which will act as an inhibitor for an enzyme. if i have used all current information about the mechanism of this enzyme to design this inhibitor and i carefully engineer it with similar chemical properties of the transition state, what type of inhibitor am i attempting to engineer and how will i know if i have succeeded?
a. an uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in vmax b. a competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in vmax c. an uncompetitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in km d. a competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in km

d. A competitive inhibitor, collect kinetic daa both in the presence and absence of inhibitor and watch for a change in Km.

Explanation:

According to the description, the inhibitor was designed in a way when it binds the enzyme, the transition state achieved will be close to the one observed when the substrate binds.

In other words, the aim was to design an inhibitor that will bind the free enzyme in a reversible way, competing with the substrate for the binding sites.

As the inhibitor will be binding the same site as the substrate, the apparent affinity of the enzyme for the substrate will decrease. And the higher the affinity of the inhibitor for the enzyme, stronger the effect it will have over the affinity of the enzyme for the substrate.

Quantitatively speaking, the apparent Km of the enzyme for the substrate will increase with the inhibitor concentration, as the affinity of the enzyme for the substrate decreases.

In summary, what is being engineered is a competitive inhibitor, and the way of knowing if the design was successful, is collecting kinetic data in presence and absence of inhibitor, and watch for changes in the apparent Km.

a mercury barometer is a device that uses the physical properties of mercury in a tube to measure barometric pressure. mercury barometers are used for : -

1. measurement of atmospheric pressure  

2. determining the altitude of a place

3. weather forecasting

4. calibration and checking of aneroid barometers

5. application in fluid mechanics

6. measurement of pressure in aircrafts

7. surface weather analysis

8. preparation of barographs

9. usage in physical sciences like physics, astronomy & chemistry

10. preparation of aircraft altimeters

characteristics of mercury barometer : -

1.used to define and measure atmospheric pressure.

2.on the average at sea level the column of mercury rises to a height of about 760 mm.

3.this quantity is equal to 1 atmosphere.

4.it is also known as standard atmospheric pressure.

5.a barometer in which the weight of a column of mercury in a glass tube with a sealed top is balanced against that of the atmosphere pressing on an exposed cistern of mercury at the base of the mercury column, the height of the column varying with atmospheric pressure.

percent abundance for cu-63 can be shown as:

63 x + 65 (1-x) = 62.9296

65 - 62.9296 = 2 x

x = 1.035

thus, percent abundance for cu-63 is 1.035

percent abundance for cu-65 can be shown as:

63 x + 65 (1-x) = 64.9278

65 - 64.9278 = 2 x

x = 0.0361

thus, percent abundance for cu-63 is 0.0361

the mass spectrum of cu-63 showing the intense peak as, this isotope is found in nature more abundantly around 92.38 % in comparison to cu-65 which is found 62.58 % naturally