Aresearcher claims that increasing temperature by 10 degrees will double the rate of an enzyme-catalyzed reaction. to test this, he designed an experiment. for each tube, the researcher will measure the rate of product formation

which best justifies the inclusion of tube 5 as a control?

it will provide a measurement of product formation in the absence of the substrate

it will provide a measurement of product formation in the presence of a denatured enzyme

it will show the effect of doubling the amount of substrate on the rate of product formation

it will show the effect of increased enzyme activity on the rate of product formation