Use henderson-hassalbach equation. the pka of tris-hcl is 8.1. after you have added 1n hcl to adjust the ph of the tris solution to 8.1 what is the ratio (in mmols) of tris to tris-hcl in the resulting buffer solution?

Explanation:

According to the Henderson-Hassalbach equation for pH is as follows.

        pH =

It is given that the pH and are same.

Hence,    

        = 0

      =

                                 = 1

Hence, whenever the pH and are same then the ratio of conjugate base to acid is 1.

Thus, we can conclude that the ratio of Tris to Tris-HCl is 1.

a. energy is absorbed in the reaction.

if in a given reaction the energy of products is greater than the energy of reactants it means that reaction is endothermic and during the reaction, the energy is absorbed by reactants. in an endothermic reaction, the products have more stored chemical energy than the reactants. and in this reaction the reactants are more stable than products before the compounds with less energy are always more stable.