Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine:
Ace...
Chemistry, 20.02.2020 18:04 inesdelude
Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine:
Acetylcholine + H2O ? acetate + choline
The Km of acetylcholinesterase for its substrate acetylcholine is 9.0 x 10-5M. In a reaction mixture containing 5 nmol/mL of acetylcholinesterase and 150.0 ?M acetylcholine, a velocity, Vo = 40.0 ?mol/mL*sec was observed for the acetylcholinesterase reaction.
A. Calculate Vmax for this amount of enzyme.
B. Calculate kcat for acetylcholinesterase.
C. Calculate the specificity constant for acetylchoinesterase.
Answers: 2
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