1. (2pts) The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.25L elution buffer for all the students this week given 500ml of 1M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and 350ml of 1M imidazole (68.08 g/mole). Show your calculations for credit.

The last few miles of the marathon are the most difficult for heather, her hair plastered to her head, sweat clinging to her arms, and her legs already feeling as if they had nothing left, just dead weight. after grabbing a cup of ice water, she feels the ice cubes smash against her nose as she gulps some cool refreshment and keeps on running. in these last few miles, the breeze kicks up and she finally feels some coolness against her skin. drips of sweat, once clinging to her forehead, now spill down, and heather feels more pain as the sweat flows into her eyes.which of the following is the most likely reason why the ice struck heather’s nose when she took a drink? a) water can function as a solvent. b) water can store large amounts of heat. c) water can moderate temperatures through evaporative cooling. d) the density of water decreases when it freezes. e) water has a cohesive nature.sweat remained on heather’s forehead and arms because of the a) high salt content of sweat b) cohesive nature of water c) ability of water to moderate heat d) high evaporative cooling effect of water e) ability of water to act as a solvent

How might this scientific phenomena be explained? a paper clip floats on water.

The empirical formula of a compound is ch2o and its mass is 120 amu/molecule, what is its formula?