Jacqueline isolated an interesting new protein from the broth of her yeast culture. After purification of the protein, she requested an aminoacid analysis and found that the protein contained 0.73% tryptophan by weight (tryptophan M 204) (a) Calculate the minimum molecular weight of the protein (i. e., assuming there is only one tryptophan residue per protein molecule). Number minimum ML b Size-exclusion chromatography of the protein gave a molecular weight estimate of 53500. How many tryptophan residues are present in a molecule of the new protein? (Enter a whole number.) Number of Number tryptophan residues

- decrease the number of plants and animals living in the water.

answer : (a) volume of the gas = 8.67 ×   l

                (b) second virial coefficient b = -1.21226 l/mole

solution :   given,     t = 300k

                                p = 20atm

                                z = 0.86

                                n = 8.2 mmoles = 8.2 × moles

                                r =   0.082 l atm/k/mol

        find,     (a) volume of gas   and (b) second virial coefficient b at 300k.

formula used :            

                          and            

(a)            

                    = \times 0.082\times 300}{20}[/tex]

                    = 8.67 ×   l

(b)      

                    = (0.00867-1) × 8.659

                    = - 8.5839 l/mole