What is meant by a calibration curve for a colored substance being studied with a spectrophotometer? How is a calibration curve used to determine the concentration of a solution of unknown concentration of the colored substance?

The concentration of the analyte is determined by fitting the absorbance or transmittance obtained by spectrophotometric analysis of the unknown solution into the calibration curve.

Explanation:

In a calibration curve, the instrumental response (absorbance or transmittance), is plotted against the concentration of the analyte (the substance to be measured). The analyst is expected to prepare a series of standard solutions of the analyte within a range of solution concentrations close to the expected concentration of analyte in the unknown solution. The method of least squares may be used to determine the best fit of the line, thus, the concentration of the analyte. This method is only used for the determination of the concentration of coloured substances (spectrophotometry).

the law of conservation of mass states that in a chemical reaction, the total mass of reactants is equal to the total mass of products. because atoms are only rearranged in a chemical reaction, there must be the same number of sodium atoms and chlorine atoms in both the reactants and products

answer

fusion: the energy source of stars. the energy released from the collapse of the gas into a protostar causes the center of the protostar to become extremely hot. when the core is hot enough, nuclear fusion commences. fusion is the process where two hydrogen atoms combine to form a helium atom, releasing energy.

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