Put these steps in the mechanism of chymotrypsin catalysis in order from first to last. Note that some references may not break out each of these steps individually, but all steps should be ordered. There will be eight steps. A. His 57 catalyzes removal of H from Ser 195 hydroxyl. B. His 57 donates H to N of scissile peptide bond, tetrahedral intermediate decomposes. C. The portion (N-terminal end) of the original substrate with the new carboxylate terminus diffuses away. D. Water donates H to His 57. E. Resulting OH attacks carbonyl of the remaining substrate. F. His 57 donates H to Ser 195 O, leading to the collapse of tetrahedral intermediate. G. the portion (the C-terminal end) of the original substrate with the new amino terminus diffuses away. H. Ser 195's nucleophilic O attacks carbonyl C of the substrate.

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The answer to your question really depends on three things: how much water you have, how cold it is to start out, and how cold the things around it are. water actually freezes when it gets to 32 degrees fahrenheit (0 degrees celsius), but the time it takes to get there may be different.  let's start with the first. if you take two glasses, and fill one with a tiny bit of water, and the other about halfway, then put them both in the freezer, the one with less water will freeze first (you can try this at home, but i recommend using plastic cups and not glass ones).  now let's move on to the second part. let's say you have two glasses, and you fill one with really cold water that has been in the refrigerator, and the other with really hot water from the sink. if you put both of them in the freezer, the one that started out colder will freeze first.  for the third part, let's imagine that you have two glasses with the same amount of water in them, and the water is at the same temperature. imagine putting one outside on a really really cold day in georgia, and having a friend in alaska put one outside on the same day. since it would be so much colder in alaska, the glass of water there would freeze before yours.  so, if you took a tiny bit of really cold water in a glass, and put it outside on a cold day in alaska, it would freeze a lot faster than a big glass of hot water outside on a cold day in georgia.