You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 4.0 mL to the 99 mL.

A.) What was your intended dilution factor?

B.) What was your actual dilution factor?

A.) Intended dilution factor : 0.01

B.) Actual dilution factor: 0.039

Explanation:

Dilution factor(DF) is ratio of the volume of the undiluted sample to total volume of diluted solution

A.) What was your intended dilution factor

This would be the the addition 1.0ml from 4.0ml to 99ml of sterile

Volume of sterile diluent = 99ml

Volume of Undiluted sample = 1.0ml

Total volume of diluted solution = 99ml + 1m = 100ml

Dilution factor = Volume of undiluted sample ÷ Total volume of diluted solution

Dilution factor = 1:100

= 1ml ÷ 100ml

= 0.01

B.) What was your actual dilution factor?

This would be the entire addition of 4.0ml to 99ml sterile diluent

Volume of sterile diluent = 99ml

Volume of Undiluted sample = 4.0ml

Total volume of diluted solution = 99ml + 4.0ml = 103ml

Dilution factor = Volume of undiluted sample ÷ Total volume of diluted solution

Dilution factor

= 4:103

= 4ml ÷ 103ml

= 0.039

in chemistry, adhesion and cohesion are attractive forces that exist between molecules. they hold the molecules together from each other. they are different in that adhesive force exists between molecules of different substance while cohesive force happens between molecules of same substance.

adhesion between water and glass causes water molecules at the edge of water and glass interface to climb up the wall in a narrow glass tube. this is called capillary action. cohesion is what gives glass its shape and causes water to form a droplet.

i think   the answer is c hope i'm right