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Chemistry, 11.03.2020 23:05 djennings8055

Match the steps for preparing a column for column chromatography. A. Add 5-6 grams of acidic alumina to the column by pouring alumina in top through petroleum ether over a 10 minute period (wash any alumina from sides of column with more petroleum ether). Make sure top of alumina layer is level by tapping on side of column. B. Add 100 mg of acetylferrocene/ferrocene solution with a Pasteur pipette to top of column without disturbing the sandC. Add sand (pouring through the petroleum ether in column) to make a 1 cm flat and level layer of sand on top of the glass wool, tapping side of column to level surfaceD. All of your compound should be in the alumina (you should see an orange-yellow band of compound right on top of the alumina column (but you should see any remaining colored solution in the sand); if all of the sample is on the alumina, fill up the column with petroleum ether (if you note any yellow color to the solution above the sand as you fill that means you didn't get all of your sample onto the alumina before filling)E. Allow petroleum ether to drain through column (filling column with more petroleum ether as needed); you will note two bands splitting from the top of alumina column, one yellow-orange band will be moving much faster and approaching bottom of column, while the other band stays near the top. F. Fill column about 1/3 up with petroleum etherG. Gather glassware, silica, sand, glass wool, solvent and sample prior to assembling the columnH. Insert a very small (but not tightly packed) amount of glass wool right above stopcock and clamp column to the ring standI. Open stopcock and drain petroleum ether till it is just above the top layer of sand (NEVER LET THE ALUMINA RUN DRY), then close stopcockJ. Open stopcock until the sample has passed into the sand layer and the level of liquid is just at the sand layer/alumina interface; add 2-4 mL of petroleum ether (without disturbing levelness of sand layer); drain this until the liquid is right at the sandlayer/alumina interface; add another 2-4 mL of petroleum ether and again drain to the interfaceK. Pour in sand to create a 1 cm level layer on top of the aluminaL. When the first band approaches the bottom of column, replace flask or beaker under column with tared flask, keep collecting solution in that flask till all of the first yellow-orange band has been recovered; put back the original beaker/flask and switch solvent to a 1:1 petroleum ether; diethyl ether solution; when second band is near bottom, switch to your other tared flask and collect all of this second band in the second tared flask. STEP 1STEP 2STEP 2STEP 3STEP 4STEP 5STEP 6All the way to STEP 12.

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