Consider a second experiment: Graduate students repeated the above experiment with one modification. The same protocol was used for the LB ampicillin plate, plating 100 microliters of the reaction mix directly to the LB amp plate. However, they modified the protocol for the LB only plate by diluting the transformation mix by 1/1000 before plating. They then plated 100 microliters of this 1/200 dilution on the LB only plate. When viewing the colonies (after 2 days of growth), they obtained the following results: