When considering free energy change, biochemists usually define a standard state, the biochemical standard state, which is modified from the chemical standard state to fit biochemical applications. Determine which of the phrases describe the biochemical standard state, the chemical standard state, or both
a. ΔG (Some text use ΔG)
b. ΔG
c. Temperature is 25
d. Constant value of Mg2+
e. PH7
f. Intial concntration of reactants and products is 1M
g. Presurre is 1 atm.
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A3.37-mg sample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0 ml. then 10.0 ml of this solution was placed in a 50-ml volumetric flask and treated with 5 ml of phenol solution plus 2 ml of sodium hypochlorite solution. the sample was diluted to 50.0 ml, and the absorbance at 625 nm was measured in a 1.00-cm cuvette and found to be 0.486. for reference, a standard solution was prepared from 10.0 mg of nh4cl (molar mass = 53.49 grams/mole) dissolved in 1.00 l of water. then 10.0 ml of this standard was placed in a 50-ml volumetric flask, treated in the same manner as the unknown, and the absorbance found to be 0.323. finally, a reagent blank was prepared using distilled water in place of unknown, it was treated in the same manner as the unknown, and the absorbance found to be 0.076. calculate the weight percent of nitrogen in the protein.
Answers: 1
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Why do cells appear different in distilled water than they do in 10% salt water?
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When a scientific theory has been tested and proved by the scientific community, it becomes a law
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When considering free energy change, biochemists usually define a standard state, the biochemical st...
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