Explain why the ability of PLP to catalyze an amino acid transformation is greatly reduced if the OH substituent of pyridoxal phosphate is replaced by OCH3. Explain why the ability of PLP to catalyze an amino acid transformation is greatly reduced if the substituent of pyridoxal phosphate is replaced by . One of the steps in all amino acid transformations is removal of a hydrogen atom from the OH substituent of pyridoxal phosphate. One of the steps in all amino acid transformations is removal of the OH substituent of pyridoxal phosphate. The hydrogen of the OH substituent forms a hydrogen bond with the nitrogen of the imine linkage. g