The Ka values for several weak acids are given below. Which acid (and its conjugate base) would be the best buffer at pH 3.7?
a. MES: Ka 7.9 x 10
b. HEPES; Ka 3.2 x 103
c. Tris; Ka 6.3 x 109
d. Formic acid: K 1.8 x 10
e. Acetic acid: K 1.8 x 10
Abbreviations:
MES, 4-Morpholineethanesulfonic acid
HEPES; 4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid
Tris, 2-Amino-2-hydroxymethyl-propane-1,3 -diol
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