To run a thin layer chromatography experiment with a chemical substance, begin by marking a horizontal line near the bottom of a TLC plate with. Place aspot of the substance onto the line. For the mobile phase, add a small amount of at the bottom of a TLC chamber. Place the plate in, thenthe chamber. Once the mobile phase approaches the top of the plate, remove the plate and mark the line. Note the position of the spots and calculate Rf values if needed.

TLC is thin-layer chromatography, a chromatography technique which is used for separating the non-volatile mixtures.

Explanation:

To run a thin layer thin layer chromatography experiment with a chemical substance, begin by marking a horizontal line near the bottom of TLC plate with PENCIL. Place a SMALL spot of the substance onto the line. For the mobile phase add a small amount of SOLVENT at the bottom of TLC chamber. Place the plate in, then COVER the chamber. Once the mobile phase approaches the top of the plate, remove the plate and mark the SOLVENT line. Note the positions of the spot and calculate the Rf if needed.