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Chemistry, 28.11.2020 01:40 IsabelAyshi
Under alkaline conditions (0.1 M KOH) the molar absorptivities of the amino acids Trp and Tyr at 240 and 280 nm are given. A 10 mg sample of the protein glucagon is hydrolyzed and diluted into 100 mL of 0.1 M KOH. For a 1 cm path length cell, the absorbance of the solution is 0.717 at 240 nm and 0.239 at 280 nm. What is the concentration of Trp? tyrosine €240 = 11,300 M-7 cm-1 €280 = 1500 M-7cm-1 tryptophan €240 = 1960 M-1 cm-1 €280 = 5380 M-1 cm-1
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Under alkaline conditions (0.1 M KOH) the molar absorptivities of the amino acids Trp and Tyr at 240...
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