List two different ways you could change the buffer to elute the bound protein(s) and achieve proper separation of the proteins. Select all that apply. Please explain why. a) add a buffer with pH=8.0

b) add a buffer with pH=5.0

c) decreasing the ionic strength of the buffer

d) increasing the ionic strength of the buffer

alkaline metals are lithium, sodium, rubidium, potassium, cesium and francium.

alkali metals have electronegativity from 0.7 to 1, lowest in periodic table of elements, which means that alkali metals (i group in periodic table) has positive oxidation number in compounds.

electronegativity is a chemical property that describes the tendency of an atom to attract a shared pair of electrons towards itself.

alkaline metals (far left in main group) have lowest ionizations energy and easy remove valence electrons,

for example, potassium is the alkali metal and has a single valence electron in the outer electron shell.

electron configuration of potassium is: ₁₉k 1s²2s²2p⁶3s²3p⁶4s¹.

they can not exist alone in nature, because they are very reactive.

the answer would be a

yes

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