Chemistry, 20.02.2021 09:30 thedocgalloway
Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The electric charge on the functional group is determined by the relationship between its pKa and the pH of the solution.
(a) Histidine has three ionizable functional groups. Draw the structure of histidine in each ionization state. What is the net charge on the histidine molecule in each ionization state?
(b) Draw the structures of the predominant ionization state of histidine at pH 1, 4, 8, and 12. Note that the ionization state can be approximated by treating each ionizable group independently.
(c) What is the net charge of histidine at pH 1, 4, 8, and 12? For each pH, will histidine migrate toward the anode (+) or cathode (-) when placed in an electric field?
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