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Which part of a feedback mechanism is able to monitor the conditions outside of cells and usually uses nerve cells to relay this information to an intergrating center

Why doesn't heat added to water make the tempature rise above 100c

Imagine that twenty i.u.’s of enzyme z were catalyzing the above reaction for one minute, under vmaxconditions, in a 3.00 ml assay volume. the assay is buffered with 20 mm phosphate buffer, ph 7.60. what will the ph be at the end of that one minute?