2.5 Nakula investigated the effect of heat on amylase. Amylase is an enzyme
that makes starch molecules break down into sugar molecules.
P
• Nakula put some amylase solution into two boiling tubes,
P and Q
• He boiled the solution in tube P. He did not heat tube Q.
• He waited until the solution in tube P had cooled down to
room temperature.
• He added equal volumes of starch solution to tube P and
boiled amylase amylase
• After 10 minutes, he tested both tubes for sugar.
and starch and starch
• Nakula found that there was sugar in tube Q. but not in tube P.