If you run three spots on a TLC plate in a 50:50 hexanes:diethyl ether mobile phase and the Rfs of the three spots are 0.23, 0.25 and 0.21. What further steps could you take to verify that the three substances are different compounds and not just a single compound with slightly varying results? Be sure to discuss structures of the two solvents in terms of their intermolecular forces.
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If you run three spots on a TLC plate in a 50:50 hexanes:diethyl ether mobile phase and the Rfs of t...
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