Chemistry, 03.09.2021 16:10 Anonymouslizard
The purification of a recombinant protein is carried out starting with 100 liters of a clarified cell lysate (i. e., the cells have been lysed, and the cell debris has been removed to give a clarified solution), which has a total protein concentration of 0.36 mg/ml and a recombinant protein concentration of 2.2 U/ml, where U denotes units of biological activity of the recombinant protein. It is known that the completely pure recombinant protein has a specific activity of 40.0 U/mg. Purification is continued until a chromatography step that yields 2.0 liters of a fraction containing the protein, with a total protein concentration of 1.11 mg/ml and a recombinant protein concentration of 43.2 U/ml. For the recombinant protein, calculate the starting and ending purity, the starting and ending specific activity, and the percentage yield and fold purification through the chromatography step
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The purification of a recombinant protein is carried out starting with 100 liters of a clarified cel...
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