Fastqc: check if the input fastq file(s) are sanger/illumina 1.9 encoding fastq groomer: reformat fastq file(s) to sanger/illumina 1.9 encoding if necessary fastq trimmer or trimmomatic: remove low quality regions alignment (e. g. bowtie2/bwa/hisat): align reads to a reference genome, giving bam/sam files sam-to-bam: convert the output file(s) to bam files if the output from the last step is a sam file variant calling (e. g. freebayes): call variants using output bam file and reference genome, giving vcf file

if you suspect that your immediate supervisor, department manager, or company is engaging in something unlawful you must do your best to end the misconduct immediately while also protecting yourself, your livelihood, and your family’s future.

a  social news website  is an  internet  website  that features  user-posted stories. such stories are ranked based on popularity, as voted on by other users of the site or by website administrators. users typically comment online on the news posts and these comments may also be ranked in popularity. since their emergence with the birth of  web 2.0, social news sites have been used to link many types of information, including news, humor, support, and discussion. all such websites allow the users to submit content and each site differs in how the content is moderated. on the  slashdot  and  fark  websites,  administrators  decide which articles are selected for the front page. on  reddit  and  digg, the articles that get the most votes from the community of users will make it to the front page. many social news websites also feature an online comment system, where users discuss the issues raised in an article. some of these sites have also applied their voting system to the comments, so that the most popular comments are displayed first. some social news websites also have a  social networking  function, in that users can set up a user profile and follow other users' online activity on the website.