- What information do you obtain from the double digest XhoI/AlwNI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert in the correct orientation (how many bands, what are their sizes)? - What is your expectation if your plasmid has an insert in the incorrect orientation (how many bands, what are their sizes)?

answer: when the substance contain the two parts (polar and non-polar) like soap.

explanation:

soap is produced from the basic hydrolysis of ester "saponification", it is considered as a salt of hydrocarbons. the soap molecule contains the two parts: a polar group   (-coo-na+) and a non-polar group (r-hydrocarbon part). the polar group is called the head and the tail is the non-polar group. the polar group is hydrophilic in nature (water loving) and the non-polar group is hydrophobic in nature (water repelling).the polar/non-polar nature of a substance is useful that it can dissolve the non-polar substances by the polar part and also can dissolve the polar substances by the non-polar part. it commonly used in detergents and soaps.

True i belive sorry if i am wrong