How efficient would pcr be if we set the annealing temperature higher or lower than the calculated melting temperature? how would a higher or lower temperature affect the annealing capability of the primers and the final quantity or quality of the products? how would a gel of such pcr products look compared to pcr products obtained at the optimum annealing temperature?

Line joining liquid phase with liquid and solid phase mixture is known as: a) liquidus b) solidus c) tie line d) none of the mentioned

Asingle-geared blanking press has a stroke of 200 mm and a rated capacity of 320 kn. a cam driven ram is assumed to be capable of delivering the full press load at constant force during the last 15 percent of a constant-velocity stroke. the camshaft has an average speed of 90 rev/min and is geared to the flywheel shaft at a 6: 1 ratio. the total work done is to include an allowance of 16 percent for friction a) estimate the maximum energy fluctuation b) find the rim weight for an effective diameter of 1.2 m and a coefficient of speed fluctuation of 0.10

At 12 noon, the count in a bacteria culture was 400; at 4: 00 pm the count was 1200 let p(t) denote the bacteria cou population growth law. find: (a) an expression for the bacteria count at any time t (b) the bacteria count at 10 am. (c) the time required for the bacteria count to reach 1800.