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Engineering, 10.12.2019 01:31 ForbesDrew

Fluorescein is a small fluorescent tracer molecule that is used in a wide variety of physiologic studies. in the eye, it is used in a technique known as fluorophotometry to characterize the transport characteristics of aqueous humor, the fluid that fills the anterior chamber behind the cornea. a drop of fluorescein (50 0.005% by mass, fluorescein in saline) is placed onto the cornea and spreads evenly over the corneal surface to create a thin film. it the diffuses through the cornea = 10−6 cm2 / and enters the aqueous humor on the back side of the cornea. the thickness of the cornea is about 0.05 cm and its radius is 0.5 cm. a) estimate how much fluorescein will enter the cornea during the first minute after application b) estimate how long it will take before a detectable quantity of fluorescein (0.0001%) begins to enter the aqueous humor

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