Exactly 5.00 ml aliquots of a solution containing phenobarbital were measured into 50.00ml volumetric flasks and made basic with koh.
the following volumes of a standard solution of phenobarbital containing 2.00 µg/ml of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.00, 0.500,1.000, 1.50, and 2.00ml.
the fluorescence of each of these solutions was measured with a fluorometer, which gave volumes of 3.26, 4.80, 6.41, 8.02, and 9.56 respectively.

(a) pot the data.
(b) using the plot from (a) calculate the concentration of phenobarbital in the unknown.
(c) derive a least-squares equation for the data.
(d) find the concentration of phenobarbital from the equation in (c)
(e) calculate a standard deviation for the concentration obtained in (d)