8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Apreheater involves the use of condensing steam at 100°c on the inside of a bank of tubes to heat air that enters at 1 atm and 25°c. the air moves at 5 m/s in cross flow over the tubes. each tube is 1 m long and has an outside diameter of 10 mm. what is the minimum value of nl needed to achieve an outlet temperature of t 75 c? what is the corresponding pressure drop across the tube bank?