Problem 1. You are operating a batch bioreactor. At the stationary phase, you turn off the oxygen supply for 5 minutes, and then turn it back on. You obtained the following data from your experiment. What is the oxygen uptake rate and kla for your experiment? (Assume the solubility limit (C*) is 7.3mg/L and use graphing method to find kla)

Time (min) DO(mg/L)
1 3.3
Air off 0 3.3
1 2.4
2 1.3
3 0.3
4 0.1
Air on 5 0
6 0.3
8 1
9 1.6
10 2
11 2.4
12 2.7
13 2.9
14 3