Which sentence segment contains a punctuation error? during rush hour yesterday, horns honked, tires screeched a whistle sounded, and shrill fire sirens moaned.

The sentence segment that contains a punctuation error is “a whistle sounded” since there is no comma after the word “screeched” and before the group of words "a whistle sounded"  The correct punctuation in the sentence should be “During rush hour yesterday, horns honked, tires screeched, a whistle sounded, and shrill fire sirens moaned.”

A Comma is a punctuation mark used to separate words or group of words within a sentence.