Loss.
Explanation:
William Shakespeare's "Hamlet" revolves around the theme of revenge, insanity and the dilemma of good over evil. And through the character of the young prince Hamlet trying to exact revenge for the death of his father King Hamlet, Shakespeare delved right into the souls of men in their agony on what to do.
Ophelia is the daughter of Polonius, and Hamlet's lover. But due to the death of her father who was unintentionally killed by Hamlet, she became insane. The song that she sang in Act IV all talk about loss, the loss of a husband, a lover or even one's virginity. She sang of a man who "is dead and gone, lady, He is dead and gone". She also sang of a man who had promised to marry her
"Before you tumbled me,
You promised me to wed.”
He answers,
“So would I ha' done, by yonder sun,
An thou hadst not come to my bed.”
The lines are suggestive of her loss of virginity to Hamlet, which is in conflict with the same issue of keeping her honour intact which her father and brother had previously warned her to be careful about. The short yet interrupted song all talk about the theme of a loss, a loss of someone or something.