Which of the following was an ecological result of european exploration from 1400 to 1800?

I couldn't find choices in Google though I looked carefully so i would say from my own research one of the very most important discoveries was of Alexander Von Humboldt and his sidekick, Aime Bonpland that species change significantly with altitude a discovery made while climbing to near the top of some very high volcanoes in Ecuador and this initiated the science of ecology ie the effect of altitude on ecology.

Ctrust me i know its c