A stock dye solution of 100 g/L has been prepared for you. You have 50 mL volumetric flasks available to prepare a series of standard solutions. Calculate the volume of stock solution that would be added to each flask to prepare standards of 0, 25, 50, and 75 g/L.

Use the law of conservation of mass to perform the calculation for the dilution:

for 0 g/L

  MdilutionVdilution = MstockVstock

   (0.0 g/L)(50 ml) = (100.0 g/L)(x ml)

   x = [(0.0 M)(50 ml)]/100.0 g/L

   x = 0 ml of stock solution

To make your solution, pour 0 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.

for 25 g/L

(25.0 g/L)(50 ml) = (100.0 g/L)(x ml)

   x = [(25.0g/L)(50 ml)]/100.0 g/L

   x = 12.5 ml of stock solution

To make your solution, pour 12.5 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.

for 50 g/L

(50.0 g/L)(50 ml) = (100.0 g/L)(x ml)

   x = [(50.0g/L)(50 ml)]/100.0 g/L

   x = 25 ml of stock solution

To make your solution, pour 25 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.

for 75 g/L

(75.0 g/L)(50 ml) = (100.0 g/L)(x ml)

   x = [(75.0g/L)(50 ml)]/100.0 g/L

   x = 12.5 ml of stock solution

To make your solution, pour 37.5 ml of stock solution into a 50 ml volumetric flask. Dilute it with solvent to the 50 ml line.

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