The map shows the spread of religions across Europe in the 1500s. Based on the map, which had occurred by 1560?

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The answer you're looking for is washing your hands. "at this late date, we all expect our doctors to wash their hands before examining us or performing an operation in order to prevent the spread of infection. surprisingly, physicians did not begin to acknowledge the lifesaving power of this simple act until 1847.it was then that dr. semmelweis began exhorting his fellow physicians at the famed vienna general hospital (allgemeines krankenhaus) to wash up before examining women about to deliver babies. his plea was far more than aesthetic; it was a matter of life and death and to prevent a deadly malady known as “childbed” or puerperal (from the latin words for child and parent) fever." 

Pretty much everywhere: walking requires friction, driving around curves, steering a car with the steering wheel, starting fire with a stick, objects moving along a conveyer belt.