What are the three limitations of an ELISA

Enzyme-linked Immunosorbent Assay, also know as ELISA are used to determine if an antibody is present in a patient's blood sample. They are found in bodily fluids.

Explanation:

The three important limitations are:

1) A positive result confirming a presence of an antibody but it not necessarily making the patient sick

2) A false negative where the amount of antibodies is too low to be measured

3) Another positive result may occur if an unrelated antibody reacts with the antigen non specifically.

a, b, c - sides of a triangle

a + b > c

a + c > b

b + c > a

we have a = 5m and b = 11m. substitute:

5 + 11 > c

16 > c → c < 16

5 + c > 11         subtract 5 from both sides

c > 6

11 + c > 5         subtract 11 from both sides

c > -6

c < 16 and c > 6 and c > -6

thereofre your answer is 6m < c < 16m

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