Of the 500 settlers who came to Jamestown before John Rolfe, how many were still alive when he arrived?

We have passenger lists for the first colonists to journey to Jamestown. The very first in 1607 included 144 men and boys, of whom 104 remained in the new colony. Only 38 were still alive when the first supply ship arrived in 1608. The First Supply arrived with no women passengers.

so 38 passengers were left

Explanation:

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