Of the various events that led to division between the colonists and Parliament which do you think was the most significant?

Personally, I would say the Seven Years War. It started the divide in the first place. The war itself costed Great Britain a great amount of money, and to make it up, they started taxing the colonists heavily. This first instance of taxation is what led to the divide, and the taxation started because of the war. Therefore, I believe that that is the most significant event.

Explanation:

immigrants from northern and western europe continued coming as they had for three centuries, but in decreasing numbers