What mistake did Pasteur's assistant make? He didn't refrigerate his cultures.
He waited longer than usual to check for results.
He injected too much of the culture.
He used an old culture of germs.

He used an old culture of germs.

Explanation:

The chickens did not become ill. Pasteur reasoned the factor that made the bacteria less deadly was exposure to oxygen.

He waited longer than usual to check for results.

Explanation:

In 1879, Pasteur observed, by chance, that old bacterial cultures lost their virulence. He had instructed an assistant to inject the chickens with a fresh culture of the viral bacteria before a holiday. The assistant, however, forgot and went on vacation.

Certified answer

radioactive dating, also known as  radioisotope dating  is a process that in  estimating the approximate  age  of  rocks. these data can be analyzed from  the studies dealing with the  decay of the radioactive elements that are present in these rocks. in nature, there are a few types of materials or atoms which are quite unstable and they spontaneously decay or breakdown to form other kinds of atoms.

"the sepoy rebellion was the result of many, many influences and stressors on the cultures of india living under british rule. in britain, it's called the sepoy mutiny on the indian mutiny, but in india, it's called the first war of independence.

the main reason is concerned to the british paramountcy , the belief in british   dominance in india political, economic and cultural life had been introduced in india about 1820."

"they were forced to grow crops that benefited the colonizing nations instead of feeding their own people."

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explanation: