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Physics, 24.08.2019 05:30 61497

For a protein with two ligand binding sites the binding polynomial has the general form: q=[p](1+2k1[l]+k1k2[l]2) where [p] is the unbound protein concentration and [l] is the free ligand concentration. the equilibrium constants are defined as: k1=[pl]/[p][l] and k2=[pl2]/[pl][l]
a. assume k1=k2=k=10 and [l]=0.15. calculate the fraction of sites bound fb
b. consider a model where k1=αk and k2=k . if k = 10 and [l] = 0.15 as before, calculate the binding fraction is α=0.1

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For a protein with two ligand binding sites the binding polynomial has the general form: q=[p](1+2k...
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