Consider a mixture of two chromophores a and b that have extinction coefficients & (2,) = 2.5x 104 m-'cm', & (92) = 8.2x104 m-'cm', az = 6.3x105 m-'cm', er (22) = 1.4x10° mº'cm' at two wavelengths 2, and 2,. the absorption coefficients of the mixture at the two wavelengths are u. (2.) = 1.8 cm" and 4. (22) = 3.1 cm", (a) what are the concentrations of chromophores a and b in the mixture? (b) what are the changes in the absorption coefficient of the mixture at the two wavelengths if the concentration of chromophore a is doubled and that of chromophore b is tripled? 2. a slab of thickness l = 1.5 cm contains a non-scattering solution of a 3.7 um concentration of a chromophore that has an extinction coefficient ε = 3.2x10 5 m cm (ignore the absorption of the solvent). if one side of the slab is illuminated with an intensity of 48 mw/cm², what is the transmitted intensity on the other side of the slab? 3. calculate absorbance for the following cases, transmitted intensity (l1), input intensity (lo) and, t = 11/ lo. (a) t = 0.999, (b) t= 0.50, (c) 0.20. 4. what is the average distance traveled by photons from a point source at the origin of a cartesian coordinate system (0,0,0) to point (2 cm, 2 cm, 2 cm) in an infinite medium with ma = 0.10 cm and us= 5.0 cm-1 ? how much longer is this distance with respect to the diffusion length in this medium?
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Consider a mixture of two chromophores a and b that have extinction coefficients & (2,) = 2.5x...
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