The binding of a compound to an enzyme is observed to slow down or stop the rate of the reaction catalyzed by the enzyme. increasing the substrate concentration reduces the inhibitory effects of this compound. which of the following could account for this observation?
1. the compound is a negative allosteric regulator.
2.the compound is a competitive inhibitor.
3. the compound forms a covalent bond with one of the amino acid residues needed for enzyme activity.
4. the compound reduces disulfide bonds, causing the enzyme molecules to partially unfold.
5 the compound causes a cofactor to be lost from the enzyme.