Physics, 29.02.2020 01:16 sunflowergirl6664

What are some drawbacks of electron microscopes? 3. If an object being viewed under the phase-contrast microscope has the same refractive index as the background material, how would it appear? +

Answers: 3

Show answers

Answers

Answer from: bobbye001

1. Some drawbacks of electron microscopes:

Price, size, repair, researcher learning and image artifacts arising from specimen preparation are the major drawbacks. This form of magnification is a massive, burdensome, costly piece of equipment, highly sensitive to vibration and exterior magnetic field.It must be held in an environment that is big enough to contain the microscope, as well as to secure and prevent any unwanted effect on the electrons.Upkeep includes ensuring balanced voltage supplies, electromagnetic coil / lens currents and cool water circulation so that the specimens are not destroyed or damaged by the heat released during the electrons energization process.

2. If an object being viewed under the phase-contrast microscope has the same refractive index as the background material than "it would be difficult to see because the phase contrast microscope amplifies differences in the refractive index".

Explanation:

In order to improve the comparison of transparent and colorless specimens with the light microscopy pictures, thus phase contrast is used. This allows the visualization of cells and cell elements which would be hard to see using a standard light magnification. The phase comparison does not involve the destruction, fixation or staining of cells.

Due to diffraction and scattering phenomena which exist at the edges of these objects, large, extended specimens are also quickly visualized with phase contrast optical. As light transits through one medium to another, the velocity is changed in proportion to the variations in the refractive index between the two media. Therefore, the wave is either increased or decreased in velocity whenever a coherent light wave produced by the oriented microscope filament progresses via a phase specimen with a particular thickness and refractive index.

Answer from: Quest

Cis the

Answer from: Quest

a sonic boom is a loud boom that breaks the sound barrier

explanation:

Another question on Physics

Physics, 21.06.2019 17:10

Aprojectile is launched horizontally 10m above the ground and with an initial velocity of 15 m/s. a) determine the magnitude and direction of projectile velocity at t= 1.3s b) determine the magnitude and direction of projectile acceleration at t=1.3s

Answers: 3

Answer

Physics, 21.06.2019 22:00

Air is held within a frictionless piston-cylinder container, which is oriented vertically. the mass of the piston is 0.45 kg and the cross-sectional area is 0.0030 m2. initially (state 1) the pressure of the gas is sufficient to support the weight of the piston as well as the force exerted by the atmospheric pressure ( 101.32 kpa). the volume occupied by the air within the cylinder in state 1 is 1.00 liter. one end of a spring (with spring constant k = 1000 n/m) is attached to the top of the piston, while the other end of the spring is attached to a stage that can move vertically. initially the spring is undeflected and therefore exerts no force. then the stage is then moved quasistatically downward a distance of 10.0 cm, at which point the system reaches state 2. the piston-cylinder is not insulated; rather it remains in diathermal contact with the surroundings, which are at a constant temperature of 300 k. what is the change of pressure within the container?

Answers: 3

Answer

Physics, 22.06.2019 01:00

An incident ray through the focal point of a spherical mirror will: reflect through the center of curvature. reflect back through the focal point. reflect parallel to the principle axis. reflect the same direction as the incident ray.

Answers: 1

Answer

Physics, 22.06.2019 07:40

An object's buoyant force and weight mean the same thing.a. trueb. false

Answers: 2

Answer

You know the right answer?

What are some drawbacks of electron microscopes? 3. If an object being viewed under the phase-contra...

Questions

Engineering, 02.10.2021 06:40

What is the correct expression of the internal moment in segment ab?...